Single cell sequencing technologies can currently be used to measure the genome (scDNA-seq), the DNA-methylome or the transcriptome (scRNA-seq) of each cell of a population. 5 Nowadays, traditional methods are thus referred to as bulk sequencing to distinguish them from single cell techniques. On the other hand, single cell sequencing measures the genomes of individual cells from a cell population. Its output is the “average genome” of the cell population. Traditional next-generation sequencing (NGS) examines the genome of a cell population, such as a cell culture, a tissue, an organ or an entire organism. This article explores how this technology works and what it tells us. While relatively recent, one of the most interesting ones is single cell sequencing. 4 A variety of sequencing methods have been developed for specific applications. During the next two decades, sequencers went through rapid evolution, optimizing, to different extents, parameters such as speed, accuracy, sequencing depth and read length. They have been named second-generation or next-generation sequencers to distinguish them from the first experimental ones, and allowed the sequencing of entire genomes. Single cell transcriptome sequencing (single cell RNA sequencing, single cell RNA-seq, or scRNA seq)Ĭombining single cell sequencing with other techniques and single cell technologyĬommercial DNA sequencers were first produced in the late 1990s. ![]() Isolation of cells and basic sample processing What is single cell sequencing and what is single cell RNA sequencing?
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